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    Genome Mapping of Radish (Raphanus Sativus L.) Using PCR-Based Markers magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Genome Mapping of Radish (Raphanus Sativus L.) Using PCR-Based Markers купить
    Genome Mapping of Radish (Raphanus Sativus L.) Using PCR-Based Markers
    4839 Руб
      Mapping of RAPD primers on chromosome 2A of common wheat magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Mapping of RAPD primers on chromosome 2A of common wheat купить
      Mapping of RAPD primers on chromosome 2A of common wheat
      4839 Руб
        Factors supporting growth of Raphanus sativus cv. newar in India magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Factors supporting growth of Raphanus sativus cv. newar in India купить
        Factors supporting growth of Raphanus sativus cv. newar in India
        6390 Руб
          Allelopathic effects on growth of plants induced by microorganisms magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Allelopathic effects on growth of plants induced by microorganisms купить
          Allelopathic effects on growth of plants induced by microorganisms
          4839 Руб
            Development of Molecular Markers for chromosome 5A of common wheat magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Development of Molecular Markers for chromosome 5A of common wheat купить
            Development of Molecular Markers for chromosome 5A of common wheat
            4839 Руб
              Genome of the Maltese Goat magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Genome of the Maltese Goat купить
              Genome of the Maltese Goat
              4139 Руб
                Management Of Fusarium Wilt Pathogen Of Tomato magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Management Of Fusarium Wilt Pathogen Of Tomato купить
                Management Of Fusarium Wilt Pathogen Of Tomato
                3549 Руб
                  Validation of deep sequencing data from Drosophila mauritiana magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Validation of deep sequencing data from Drosophila mauritiana купить
                  Validation of deep sequencing data from Drosophila mauritiana
                  2359 Руб
                    Detection of BHV-1 infection in breeding bulls magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Detection of BHV-1 infection in breeding bulls купить
                    Detection of BHV-1 infection in breeding bulls
                    5790 Руб
                      Varietal Evaluation of Radish on Different Sowing Methods magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Varietal Evaluation of Radish on Different Sowing Methods купить
                      Varietal Evaluation of Radish on Different Sowing Methods
                      4929 Руб
                        Genetic Dissection of Grain Weight & Related Traits in Bread Wheat magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Genetic Dissection of Grain Weight & Related Traits in Bread Wheat купить
                        Genetic Dissection of Grain Weight & Related Traits in Bread Wheat
                        6690 Руб
                          Nanoparticle Sensor to Detect and Quantify Pig DNA in Processed Foods magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Nanoparticle Sensor to Detect and Quantify Pig DNA in Processed Foods купить
                          Nanoparticle Sensor to Detect and Quantify Pig DNA in Processed Foods
                          7390 Руб
                            Genetic diversity studies of Cashew magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Genetic diversity studies of Cashew купить
                            Genetic diversity studies of Cashew
                            6390 Руб
                              Kernel Methods in Analysis of Genetic Markers and DNA Microarrays magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Kernel Methods in Analysis of Genetic Markers and DNA Microarrays купить
                              Kernel Methods in Analysis of Genetic Markers and DNA Microarrays
                              8890 Руб
                                Application of Molecular Markers in Brassica magdi mousa genome mapping of radish raphanus sativus l using pcr based markers Application of Molecular Markers in Brassica купить
                                Application of Molecular Markers in Brassica
                                4929 Руб
                                Massively parallel sequencing technology has a wide range of applications. Its use in SNP detection is already widespread and promises results of high accuracy. The aim is to validate data generated with the Genome Analyser II. SNPs, detected by using different mapping and SNP estimation parameters implemented in the bioinformatics tool CLC were compared to SNPs that are detected by using the method of conventional Sanger sequencing. Lines of D. mauritiana were used. As reference genome in CLC I was bound to use the genome of D. simulans, because there is no available genome of D. mauritiana until now. Using a stringent parameter set (short parameter set) that allows few mismatches of reads when mapping against the reference genome, only few regions are recovered which show divergence to the reference. 60%-81% SNPs detected with this parameter set are false positive ones compared to Sanger sequencing. If a less stringent parameter set is used this results in a very high number of false positive SNPs. Two times more SNPs were recovered with this parameter set, thereof 70-80% are false positive ones. Based on my results I drew conclude that the best approach for SNP detection is to make one first run permitting a high number of mismatches. The next step should be the use of more stringent values to reduce the high number of false positive SNPs.

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